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Effects of a phorbol ester on cell proliferation, cytoskeleton and chromosomes in 3T3 T proadipocytes
1Department of Pathology, University of Otago Medical School, Dunedin, New Zealand.
Abstract:
Tumour-promoting 12-0-tetradecanoylphorbol-13-acetate (TPA) showed a biphasic effect on cell proliferation of BALB/3T3 T proadipocytes. TPA (100 ng/ml) inhibited cell proliferation after 6-18 h of treatment but stimulated it during the next 3 d. Cultures treated with TPA for 24 d showed a 3.3 times higher saturation density compared with those without TPA treatment. TPA-induced morphological alterations were studied by immunofluorescence and scanning electronmicroscopy. In addition, detergent-extracted whole mount cell observations were carried out with or without immuno-electronmicroscopy. The results showed that TPA induced a rapid and reversible assembly of actin filaments and redistribution of microtubules which commenced as early as 15-20 min, but did not influence the frequency of sister chromatid exchange.