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Comparison of a New and Rapid Method: Brucella Coombs Gel Test With Other Diagnostic Tests
Fatma Kalem1, Ayşe Gül Ergün2, Süleyman Durmaz2
1Department of Microbiology and Clinical Microbiology, Konya Numune Hospital, Konya, Turkey. drfatmakalem@yahoo.com.
Background:
The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis.
Methods:
Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination tests of 78 patients with presumptive diagnosis of brucellosis which were sent to Microbiology Laboratory of Konya Numune Hospital from various regions of Konya were studied.
Results:
Of 78 patients with ELISA IgG and IgM, STA, BICA and BCGT; 26, 21, 10, 12 and 12 were positive. When compared with BICA, the sensitivity and specifity of BCGT were 100% and 100%, respectively.
Conclusion:
According to results BCGT can be used as a diagnostic test in routine laboratories after more comprehensive studies in control groups and patients.
