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Updated: Mar 24, 2026

Aerobic Biodegradation Testing of Materials Using a Natural Marine Seawater Inoculum and Closed Loop Respirometer
Published on: October 24, 2025
Anoxic biodegradation of petroleum hydrocarbons in saline media using denitrifier biogranules
Gholamreza Moussavi1, Sakine Shekoohiyan1, Kazem Naddafi2
1Department of Environmental Health Engineering, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract:
The total petroleum hydrocarbons (TPH) biodegradation was examined using biogranules at different initial TPH concentration and contact time under anoxic condition in saline media. The circular compact biogranules having the average diameter between 2 and 3mm were composed of a dense population of Bacillus spp. capable of biodegrading TPH under anoxic condition in saline media were formed in first step of the study. The biogranules could biodegrade over 99% of the TPH at initial concentration up to 2g/L at the contact time of 22h under anoxic condition in saline media. The maximum TPH biodegradation rate of 2.6 gTPH/gbiomass.d could be obtained at initial TPH concentration of 10g/L. Accordingly, the anoxic biogranulation is a possible and promising technique for high-rate biodegradation of petroleum hydrocarbons in saline media.
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