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Updated: Aug 8, 2026
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
Published on: May 6, 2018
Tectorigenin inhibits osteosarcoma cell migration through downregulation of matrix metalloproteinases in vitro
Yu Guo1, Ya-Hong Chen, Zhi-Hua Cheng
1Departments of aNeurosurgery bPlastic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Abstract:
Tectorigenin (Tec) is an effective component of the traditional Chinese medicine Belamcanda chinensis, which has been reported to exert beneficial effects in various types of cancer. However, the activity and mechanism of Tec in osteosarcoma (OS) have not been investigated to date. The aim of the present study was to examine the inhibitory effect of Tec on OS and its underlying mechanism of action. OS cells (Saos2 and U2OS) were treated with various concentrations of Tec for 24, 48, and 72 h. Cell proliferation was evaluated using an CCK-8 assay. Cell migration and invasion ability were measured using the Transwell assay. The expressions of MMP1, MMP2, MMP9, and cleaved caspase3 were measured using real-time PCR and/or western blot analysis. We found that Tec inhibited the proliferation of OS cells (Saos2 and U2OS) in a dose-dependent and time-dependent manner. In addition, Tec significantly inhibited migration and invasion in OS cells (P<0.05). Tec upregulated the expression of cleaved caspase3, while downregulating the expression of MMP1, MMP2, and MMP9. Taken together, the present study provided fundamental evidence for the application of Tec in chemotherapy against OS.
Insights
Tectorigenin (Tec) inhibits osteosarcoma (OS) cell proliferation, migration, and invasion. This study provides evidence for Tec
Area of Science:
- Oncology
- Pharmacology
- Biochemistry
Background:
- Tectorigenin (Tec) is a key component of Belamcanda chinensis, a traditional Chinese medicine.
- Previous research indicates Tec possesses anti-cancer properties across various cancer types.
- The specific effects and mechanisms of Tec in osteosarcoma (OS) remain largely unexplored.
Purpose of the Study:
- To investigate the inhibitory effects of Tectorigenin on osteosarcoma cells.
- To elucidate the underlying molecular mechanisms of Tec's action in osteosarcoma.
Main Methods:
- Osteosarcoma cell lines (Saos2 and U2OS) were treated with varying concentrations of Tec.
- Cell proliferation was assessed using the CCK-8 assay.
- Cell migration and invasion were evaluated via Transwell assays.
- Gene and protein expression levels of MMP1, MMP2, MMP9, and cleaved caspase-3 were quantified using real-time PCR and Western blot.
Main Results:
- Tec demonstrated a dose- and time-dependent inhibition of osteosarcoma cell proliferation.
- Significant reduction in migration and invasion capabilities of OS cells was observed following Tec treatment (P<0.05).
- Tec treatment led to increased expression of cleaved caspase-3 and decreased expression of MMP1, MMP2, and MMP9.
Conclusions:
- Tectorigenin exhibits significant anti-proliferative, anti-migratory, and anti-invasive effects on osteosarcoma cells.
- The observed effects are associated with the modulation of apoptosis-related proteins and matrix metalloproteinases.
- These findings support the potential of Tectorigenin as a therapeutic agent for osteosarcoma chemotherapy.
