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Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit
09:24

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

Published on: July 29, 2007

The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids

G Del Sal1, G Manfioletti, C Schneider

  • 1International Center of Genetic Engineering and Biotechnology, Trieste, Italy.

Biotechniques
|May 1, 1989
PubMed

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...