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Improved sensitivity of lateral flow assay using paper-based sample concentration technique.

Ruihua Tang¹, Hui Yang², Jane Ru Choi³

¹School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China; Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an 710072, PR China; The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, PR China.

|March 20, 2016

Summary

We enhanced lateral flow assays (LFAs) sensitivity using a dialysis-based concentration method. This novel device improves detection for Human Immunodeficiency Virus (HIV) nucleic acids and myoglobin, enabling more accurate point-of-care diagnostics.

Keywords:

Dialysis-based concentration HIV Lateral flow assay MYO Paper-based device

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Published on: November 13, 2021

Area of Science:

Biomedical Engineering
Analytical Chemistry
Point-of-Care Diagnostics

Background:

Lateral flow assays (LFAs) are valuable for point-of-care testing, particularly in resource-limited areas.
The limited sensitivity of conventional LFAs restricts their diagnostic utility and widespread adoption.
Improving LFA sensitivity is crucial for accurate and reliable detection of various biomarkers.

Purpose of the Study:

To develop an integrated device combining dialysis-based concentration with LFAs.
To enhance the sensitivity and lower the detection limits of LFAs for specific analytes.
To assess the device's potential for medical diagnostics, food safety, and environmental monitoring.

Main Methods:

Integration of a dialysis-based concentration technique into the LFA platform.
Testing the enhanced LFA device for the detection of Human Immunodeficiency Virus (HIV) nucleic acids.
Evaluating the device's performance for myoglobin (MYO) detection using clinically relevant concentrations.

Main Results:

Achieved a 10-fold signal enhancement for HIV nucleic acid detection, with a detection limit of 0.1 nM.
Demonstrated a 4-fold signal enhancement for myoglobin detection, achieving a detection limit of 1.56 ng/mL.
The integrated LFA device provided results in under 25 minutes.

Conclusions:

The novel integrated device significantly enhances LFA sensitivity through a simple, low-cost concentration method.
This approach offers a portable and effective solution for highly sensitive detection of diverse target analytes.
The technology shows promise for advancing point-of-care diagnostics, food safety, and environmental monitoring applications.