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Updated: Mar 24, 2026

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
A batch injection analysis system with square-wave voltammetric detection for fast and simultaneous determination of
Thiago da Costa Oliveira1, Jhonys Machado Freitas1, Rodrigo Alejandro Abarza Munoz1
1Instituto de Química, Universidade Federal de Uberlândia, Av. João Naves de Ávila, 2121 Uberlândia, MG, Brazil.
Abstract:
In this work, a batch-injection analysis system with square-wave voltammetric (BIA-SWV) detection was applied for the first time to the simultaneous determination of inorganic (zinc) and organic (naphazoline) species. Both compounds were detected in a single run (70 injections h(-1)) with a small injection volume (∼100 µL). The calibration curves exhibited linear response range between 3.0 and 21.0 μmol L(-1) (r=0.999) for naphazoline and between 10.0 and 60.0 μmol L(-1) (r=0.992) for zinc. The detection limits were 0.13 and 0.04 μmol L(-1) for zinc and naphazoline, respectively. Good reproducibility was achieved for multiple measurements of a solution containing both species (RSD<1.0%; n=20). The results obtained with the BIA-SWV method for the simultaneous determination of naphazoline and zinc were compared to those obtained by HPLC (naphazoline) and by FAAS (zinc); no statistically significant differences were observed (95% confidence level).
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