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Purification of mumps virus particles of high viability
Huosheng Chen1, Laurie Ngo1, Svetlana Petrovskaya1
1Food and Drug Administration, Center for Biologics Evaluation and Research, 10903 New Hampshire Avenue, Silver Spring, MD 20993, United States.
Abstract:
Mumps is a highly infectious viral disease of humans with a wide array of clinical manifestations ranging from painful swelling of the salivary glands to meningitis and encephalitis. Despite the clinical importance of mumps virus, most of what is known of its biological properties comes from studies using supernatants from virus infected cell cultures, which contain substantial levels of host cell derived debris and biologically active substances such as cytokines, transcription factors and secreted virus proteins. These contaminants complicate interpretation of studies of virus replication, virus-host interactions and in vivo virulence. Here we describe a protocol for concentration of the virus from cell culture supernatants followed by gradient purification, resulting in attainment of high titer live virus of high purity.
Insights
Researchers developed a new protocol to purify mumps virus (MuV) from cell cultures. This method yields high-titer, pure live virus, improving studies on mumps virus replication and virulence.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Mumps virus (MuV) is a significant human pathogen causing diverse clinical symptoms.
- Current research on MuV relies on virus preparations contaminated with host cell components.
- These contaminants hinder accurate interpretation of virus replication and host interaction studies.
Purpose of the Study:
- To develop a protocol for obtaining high-purity mumps virus from cell culture supernatants.
- To enable more reliable studies of mumps virus biology and pathogenesis.
Main Methods:
- Concentration of mumps virus from infected cell culture supernatants.
- Gradient purification of the concentrated virus.
- Assessment of virus purity and titer.
Main Results:
- Achieved high titers of live mumps virus.
- Obtained highly purified virus preparations.
- Significantly reduced host cell-derived contaminants.
Conclusions:
- The described protocol effectively purifies mumps virus.
- This method provides a valuable tool for future mumps virus research.
- Facilitates accurate studies on mumps virus replication, host interactions, and virulence.
