Related Experiment Videos
Expression of hepatitis B virus large envelope protein in Escherichia coli and Saccharomyces cerevisiae
E Korec1, J Korcová, Z Palková
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Folia Biologica
|January 1, 1989
Abstract:
The gene coding for hepatitis B large envelope protein was cloned under the lac promoter in bacterial vector pUC-8 and under the ADH1 promoter in yeast expression shuttle vector pVT103-U, and expression of HBsAg in bacteria and yeast was determined. The strongest expression of large envelope protein was obtained after transformation of the protease-deficient yeast strain BJ1991. The recombinant large envelope protein did not form complex 22-nm particles and was not secreted into medium.