Expression and interaction of the mango ethylene receptor MiETR1 and different receptor versions of MiERS1

Different versions of the mango ethylene receptor MiERS1 were identified and the analysis indicates that, in addition to MiERS1, two short versions of this receptor (MiERS1m, MiERS1s), representing truncated proteins with central deletions of functional domains, are present in mango. The short receptor versions reveal a different expression pattern compared to MiERS1, and they are highly variably transcribed. With transient expression assays using fluorescent fusion proteins, the localisation and the interaction of the receptors were determined in leaf cells of the tobacco model. MiERS1, MiETR1, and the short MiERS1 receptor versions are anchored in the endoplasmic reticulum (ER) membrane and co-localise with each other and with an ER-marker. Furthermore, ectopic expression of the mango receptors appears to induce a re-organisation of the ER resulting in accumulation of ER bodies. Interaction assays suggest that both short MiERS1 receptor versions can bind to proteins located in the ER. Bi-molecular fluorescence complementation (BiFC) assays indicate, that MiERS1m may dimerise with itself and can also interact with MiERS1, but not with MiETR1. Further, it as found that MiETR1 can interact with MiERS1. Interaction of MiERS1s with the other ethylene receptors could not be detected, although it was located in the ER membrane system.