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Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Selectins01:25

Selectins

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Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
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Adherens Junctions01:24

Adherens Junctions

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Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
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Intracellular Signaling Affects Focal Adhesions01:17

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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
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Related Experiment Video

Updated: Mar 24, 2026

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
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Post-translational modification-regulated leukocyte adhesion and migration.

Jia Tong Loh1, I-Hsin Su1

  • 1School of Biological Sciences, College of Science, Nanyang Technological University, Republic of Singapore.

Oncotarget
|March 20, 2016
PubMed
Summary

The Ezh2 enzyme regulates leukocyte adhesion and migration by methylating talin, a key protein. This discovery offers new insights into immune responses and diseases like cancer.

Keywords:
EZH2adhesiondendritic cellsmigrationpost-translational modifications

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Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Area of Science:

  • Immunology and Cell Biology
  • Molecular and Cellular Medicine

Background:

  • Leukocyte migration is crucial for immune responses, involving complex protein coordination.
  • Post-translational modifications (PTMs) regulate the function of proteins involved in cell migration.
  • Integrin-mediated adhesion and transmigration are key steps in leukocyte recruitment to tissues.

Purpose of the Study:

  • To review leukocyte migration mechanisms and the role of PTMs in regulating these processes.
  • To examine the specific function of Ezh2-mediated talin methylation in leukocyte adhesion dynamics.
  • To discuss the implications of this regulatory mechanism in immunity and disease pathogenesis.

Main Methods:

  • Review of existing literature on leukocyte migration and post-translational modifications.
  • Analysis of studies investigating the role of Ezh2 in integrin signaling.
  • Examination of molecular mechanisms of talin methylation and its impact on leukocyte adhesion.

Main Results:

  • Ezh2, a lysine methyltransferase, directly methylates talin, a critical regulator of integrin-cytoskeleton linkage.
  • This Ezh2-mediated methylation of talin governs leukocyte adhesion dynamics.
  • The findings highlight a novel regulatory pathway controlling leukocyte migration.

Conclusions:

  • Ezh2-mediated talin methylation is a fundamental mechanism controlling leukocyte migration.
  • This pathway has significant implications for understanding immune responses.
  • Dysregulation of this mechanism may contribute to pathogenic processes, including allergic contact dermatitis and tumorigenesis.