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Updated: Mar 24, 2026
Author Spotlight: Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures
Published on: June 23, 2023
Brain tumor modeling using the CRISPR/Cas9 system: state of the art and view to the future
Xiao-Yuan Mao1,2, Jin-Xiang Dai3, Hong-Hao Zhou1,2
1Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha, P. R. China.
Abstract:
Although brain tumors have been known tremendously over the past decade, there are still many problems to be solved. The etiology of brain tumors is not well understood and the treatment remains modest. There is in great need to develop a suitable brain tumor models that faithfully mirror the etiology of human brain neoplasm and subsequently get more efficient therapeutic approaches for these disorders. In this review, we described the current status of animal models of brain tumors and analyzed their advantages and disadvantages. Additionally, prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), a versatile genome editing technology for investigating the functions of target genes, and its application were also introduced in our present work. We firstly proposed that brain tumor modeling could be well established via CRISPR/Cas9 techniques. And CRISPR/Cas9-mediated brain tumor modeling was likely to be more suitable for figuring out the pathogenesis of brain tumors, as CRISPR/Cas9 platform was a simple and more efficient biological toolbox for implementing mutagenesis of oncogenes or tumor suppressors that were closely linked with brain tumors.
Insights
Developing advanced brain tumor models is crucial for understanding cancer causes and improving treatments. This review highlights CRISPR/Cas9 technology as a powerful tool for creating accurate brain tumor models to advance research.
Area of Science:
- Neuro-oncology
- Genetics
- Molecular Biology
Background:
- Brain tumors remain a significant health challenge with poorly understood etiology and limited treatment options.
- Existing animal models for brain tumors have limitations in accurately reflecting human disease.
- There is a critical need for improved models to study brain tumor pathogenesis and develop effective therapies.
Purpose of the Study:
- To review current animal models for brain tumors, analyzing their strengths and weaknesses.
- To introduce clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology.
- To propose CRISPR/Cas9 as a superior method for developing accurate brain tumor models.
Main Methods:
- Review of existing literature on animal models of brain tumors.
- Description of the principles and applications of CRISPR/Cas9 genome editing technology.
- Analysis of CRISPR/Cas9's potential in creating genetically modified brain tumor models.
Main Results:
- Current animal models have inherent limitations in mimicking human brain tumors.
- CRISPR/Cas9 offers a versatile and efficient platform for genome editing.
- CRISPR/Cas9 facilitates the precise mutagenesis of oncogenes and tumor suppressors relevant to brain tumors.
Conclusions:
- CRISPR/Cas9 technology presents a promising approach for establishing more accurate and relevant brain tumor models.
- CRISPR/Cas9-mediated modeling can significantly enhance the understanding of brain tumor pathogenesis.
- This technology holds potential for developing more efficient therapeutic strategies for brain neoplasms.
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