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Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Introduction to Nuclear Reprogramming01:14

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Related Experiment Video

Updated: Mar 24, 2026

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Integration-Free Reprogramming of Lamina Propria Progenitor Cells.

R A Howard-Jones1, O K Y Cheung2, A Glen2

  • 1Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, Wales, UK howardjonesRA1@cf.ac.uk.

Journal of Dental Research
|March 20, 2016
PubMed
Summary

Researchers reprogrammed oral mucosal cells into integration-free induced pluripotent stem cells (iPSCs). This minimally invasive method offers a promising source for personalized medicine and regenerative therapies.

Keywords:
IPS cellshiPSCshuman induced pluripotent stem cellsoral mucosaregenerative medicinestem cells

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Last Updated: Mar 24, 2026

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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

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Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Personalized Medicine

Background:

  • Induced pluripotent stem cells (iPSCs) are crucial for personalized medicine and therapeutic testing.
  • Identifying easily accessible, noninvasively obtained cell sources for reprogramming is clinically significant.
  • Nonintegrating reprogramming methods are essential for safe regenerative applications.

Purpose of the Study:

  • To demonstrate the reprogramming of oral mucosal lamina propria progenitor cells into iPSCs.
  • To utilize nonintegrating methods for genetic manipulation during reprogramming.
  • To establish a minimally invasive cell source for iPSC generation.

Main Methods:

  • Oral mucosal lamina propria progenitor cells were collected from patients during routine dental procedures.
  • Reprogramming was achieved using nonintegrating plasmids encoding the six core pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, cMYC).
  • Generated iPSCs were assessed for genetic integration and differentiation potential.

Main Results:

  • Successfully reprogrammed oral mucosal progenitor cells into integration-free induced pluripotent stem cells (iPSCs).
  • The resulting iPSCs exhibited pluripotency, differentiating into all three germ layers (mesoderm, ectoderm, endoderm).
  • The reprogramming process did not involve genetic integration of the vector DNA.

Conclusions:

  • Oral mucosal lamina propria progenitor cells are a viable, minimally invasive source for generating integration-free iPSCs.
  • These iPSCs hold significant potential for applications in personalized medicine and regenerative therapies.
  • The concurrent collection with routine dental treatments enhances clinical feasibility.