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Updated: Aug 1, 2026

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
Published on: February 8, 2011
Hypoglycemic effect of liposome-entrapped insulin administered by various routes into normal rats
J Petkowicz1, A Byra, T Szumiło
1Department of Biochemistry and Drug Metabolism, Medical School, Lublin, Poland.
Abstract:
We entrapped insulin into liposomes using one combination of lipids comprising egg lecithin-cholesterol-stearylamine (7:2:1 molar ratio). The efficiency of entrapment was about 20% with unsonicated liposomes (LMV), and around 5% with sonicated liposomes (SUV). LMV-, SUV- and free-insulins were administered via different routes into male, non-diabetic Wistar rats in order to change the glycemia. When administered parenterally all preparations acted in a similar manner, reducing the glycemia after 2 h in the range of 75% to 85% in case of iv administration, and 43% to 67%--after sc administration. Only liposome insulin acted via intragastric route, and SUV-insulin-via buccal--or nasal route.
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