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Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
Published on: September 10, 2014
Transcriptome analysis of phycocyanin inhibitory effects on SKOV-3 cell proliferation
Jun Ying1, Jian Wang2, Huijuan Ji2
1School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University, Wenzhou 325035, China.
Abstract:
Phycocyanin (PC) from Spirulina platensis has inhibitory effects on tumor cell growth. In this research, the transcriptome study was designed to investigate the underlying molecular mechanisms of PC inhibition on human ovarian cancer cell SKOV-3 proliferation. The PC IC50 was 216.6μM and 163.8μM for 24h and 48h exposure, respectively, as determined by CCK-8 assay. The morphological changes of SKOV-3 cells after PC exposure were recorded using HE staining. Cells arrested in G2/M stages as determined by flow cytometry. The transcriptome analysis showed that 2031 genes (with > three-fold differences) were differentially expressed between the untreated and the PC-treated cells, including 1065 up-regulated and 966 down-regulated genes. Gene ontology and KEGG pathway analysis identified 18 classical pathways that were remarkably enriched, such as neurotrophin signaling pathway, VEGF signaling pathway and P53 signaling pathway. qPCR results further showed that PTPN12, S100A2, RPL26, and LAMA3 increased while HNRNPA1P10 decreased in PC-treated cells. Molecules and genes in those pathways may be potential targets to develop treatments for ovarian cancer.
Insights
Phycocyanin (PC) from Spirulina platensis inhibits ovarian cancer cell growth by altering gene expression. This study reveals molecular mechanisms, identifying potential therapeutic targets for ovarian cancer treatment.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Phycocyanin (PC), derived from Spirulina platensis, exhibits anti-tumor properties.
- Ovarian cancer remains a significant health challenge, necessitating novel therapeutic strategies.
Purpose of the Study:
- To elucidate the molecular mechanisms by which PC inhibits human ovarian cancer cell (SKOV-3) proliferation.
- To identify potential molecular targets for ovarian cancer therapy.
Main Methods:
- Cell viability was assessed using CCK-8 assay to determine PC's IC50 values.
- HE staining was used for morphological observation, and flow cytometry analyzed cell cycle arrest.
- Transcriptome sequencing identified differentially expressed genes, followed by Gene Ontology and KEGG pathway analysis.
Main Results:
- PC inhibited SKOV-3 cell proliferation with IC50 values of 216.6μM (24h) and 163.8μM (48h).
- PC treatment induced G2/M cell cycle arrest and morphological changes in SKOV-3 cells.
- Transcriptome analysis revealed 2031 differentially expressed genes, enriching pathways like neurotrophin, VEGF, and P53 signaling. qPCR confirmed differential expression of specific genes (PTPN12, S100A2, RPL26, LAMA3, HNRNPA1P10).
Conclusions:
- PC effectively inhibits ovarian cancer cell proliferation through complex molecular mechanisms.
- The identified signaling pathways and genes represent potential therapeutic targets for ovarian cancer treatment.
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