Related Concept Videos

Neonatal Mouse Bone Marrow Isolation and Preparation of Bone Marrow-Derived Macrophages06:53

Neonatal Mouse Bone Marrow Isolation and Preparation of Bone Marrow-Derived Macrophages

This protocol describes a non-enzymatic and straightforward method for isolating 7-9-day-old neonatal mouse bone marrow cells and generating differentiated macrophages using a supernatant of L929 cells as a source of granulocyte colony-stimulating factor (M-CSF). The bone marrow-derived macrophages were further analyzed for surface antigens F4/80, CD206, CD11b, and functional...
2.8K
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology03:56

Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology

This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic...
2.7K
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow02:57

Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow

This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult...
4.7K
An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells08:39

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive...
20.4K
Homing of Hematopoietic Cells to the Bone Marrow10:40

Homing of Hematopoietic Cells to the Bone Marrow

This article describes a protocol used to study the homing of hematopoietic cells to their niches in the bone...
30.0K
Culture of myeloid dendritic cells from bone marrow precursors06:39

Culture of myeloid dendritic cells from bone marrow precursors

This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are...
23.1K