Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR

CRISPR

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Orbital-Engineered Sn/RuO<sub>2</sub> Nanocatalyst with Self-Regulating Electron Configuration for Durable Chlorine Evolution at Industrial Current Densities.

ACS applied materials & interfaces·2026

Same author

Triple-channel visualization of uric acid in human urine: A probe based on triple-emissive cyan carbon dots.

Talanta·2026

Same author

Wnt dynamics at the blastopore and stomodeum during sea urchin gastrulation.

Development (Cambridge, England)·2026

Same author

Exceeding 30% Efficiency of Red Perovskite Quantum Dot Light-Emitting Diodes via Interparticle Energy Dissipation Suppression.

Nano-micro letters·2026

Same author

Mott-Schottky Heterojunction on Self-Supporting TiO<sub>2</sub> Nanotubes Enables Efficient and Stable Chlorine Evolution Reaction.

The journal of physical chemistry letters·2026

Same author

Influence of access cavity design on instrumentation efficiency in 3D-printed replicas of mandibular incisors with Vertucci Type III canals.

BMC oral health·2026

Same journal

Proton Transfer Shuttle Mediated Dormant-Active Balance for Accelerated and Controlled Polymerization of N-Carboxyanhydrides.

Angewandte Chemie (International ed. in English)·2026

Same journal

Chloride-Regulated Depolymerization of Aluminosilicate Networks for Fast Ion Transport Compliant Interfaces in Sustainable All-Solid-State Sodium Batteries.

Angewandte Chemie (International ed. in English)·2026

Same journal

Asymmetric Zn─N<sub>2</sub>O-Coordinated Hydrogen-Bonded Organic Frameworks for Electrochemical Hydrogen Peroxide Production and Wastewater Purification.

Angewandte Chemie (International ed. in English)·2026

Same journal

Photocatalytic Cascade Nitrogen Fixation for Selective Purification of Methane-Rich Coal-Bed Gas Over a Bimetallic MOF.

Angewandte Chemie (International ed. in English)·2026

Same journal

Scalable Art-Inspired Tessellated Covalent Organic Framework Membranes Enable Highly Selective Ion Separation.

Angewandte Chemie (International ed. in English)·2026

Same journal

Layered Copper-Anthraquinone Coordination Polymer Cathode Leveraging Dual-Redox Sites and Facilitated Ion Diffusion for High-Performance Lithium-Ion Batteries.

Angewandte Chemie (International ed. in English)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 24, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Conditional Control of CRISPR/Cas9 Function.

Wenyuan Zhou¹, Alexander Deiters²

¹Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA.

Angewandte Chemie (International Ed. in English)

|March 22, 2016

Summary

This summary is machine-generated.

The CRISPR/Cas9 gene editing tool can now be precisely controlled using small molecules or light. This advancement enhances genome editing specificity and allows for spatiotemporal control in biological research.

Keywords:

CRISPR Cas9 conditional control genomic editing light small molecules

More Related Videos

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Related Experiment Videos

Last Updated: Mar 24, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

The CRISPR/Cas9 system is a powerful tool for genome editing, synthetic biology, and gene regulation.
Current applications require precise control over its activity.

Purpose of the Study:

To develop methods for conditional activation of CRISPR/Cas9.
To improve the specificity and spatiotemporal control of genome editing.

Main Methods:

Development of small molecule-inducible systems for Cas9.
Engineering light-inducible systems for Cas9 activation.
Testing these systems in cellular and animal models.

Main Results:

Achieved conditional activation of CRISPR/Cas9 using small molecules.
Demonstrated light-inducible control over Cas9 activity.
Observed enhanced specificity and precise temporal/spatial control of genome editing.

Conclusions:

Conditional activation strategies significantly improve CRISPR/Cas9 system control.
These advancements expand the utility of CRISPR/Cas9 for precise genomic modification and gene regulation.