Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ionization and Chain Size of Weak Polyelectrolytes in Semidilute Regime.

Macromolecules·2026
Same author

Modeling the Aerosol Aqueous Phase: Solvation of Pyruvic Acid Analogs and Na<sup>+</sup>, Cl<sup>-</sup> Ions.

The journal of physical chemistry. A·2026
Same author

Automatization of Atmospheric OH Radical Abstraction Reactions.

The journal of physical chemistry. A·2026
Same author

Fast and Interpretable Machine Learning Modeling of Atmospheric Molecular Clusters.

The journal of physical chemistry. A·2026
Same author

Charge Regulation Enables Uptake of Ampholytes to Polyelectrolyte Brushes.

ACS macro letters·2025
Same author

Correction to "Improved Configurational Sampling Protocol for Large Atmospheric Molecular Clusters".

ACS omega·2025
Same journal

Nanopore sequencing with proteins: synchronization and dischronization of molecular dynamics simulations with laboratory and industrial developments.

Soft matter·2026
Same journal

Catanionics from biosurfactants and regular surfactants: miscibility and structure.

Soft matter·2026
Same journal

Adhesives with a thickness smaller than the fractocohesive length enhance adhesion.

Soft matter·2026
Same journal

Non-equilibrium phase transitions in hybrid Voronoi models of cell colonies.

Soft matter·2026
Same journal

Effects of methoxy substituents on self-assembly and gelation performance of benzamide-based organogelators.

Soft matter·2026
Same journal

Rheology of <i>Escherichia coli</i> suspensions with various bacterial morphologies and motion characteristics.

Soft matter·2026
See all related articles

Related Experiment Video

Updated: Mar 24, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

12.0K

Mean squared displacement from fluorescence correlation spectroscopy.

Jakub Kubečka1, Filip Uhlík1, Peter Košovan1

  • 1Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 00 Praha 2, Czech Republic. peter.kosovan@natur.cuni.cz.

Soft Matter
|March 22, 2016
PubMed
Summary
This summary is machine-generated.

Fluorescence correlation spectroscopy (FCS) can approximate mean squared displacement (MSD) under specific conditions. This study reveals that apparent MSD from FCS often deviates from true MSD, especially for polymer diffusion, and proposes a verification method.

More Related Videos

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

12.1K
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

16.8K

Related Experiment Videos

Last Updated: Mar 24, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

12.0K
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

12.1K
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS
10:59

Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy FCS

Published on: April 6, 2012

16.8K

Area of Science:

  • Biophysics
  • Physical Chemistry
  • Polymer Science

Background:

  • Fluorescence Correlation Spectroscopy (FCS) is a technique used to study molecular dynamics.
  • Mean Squared Displacement (MSD) is a key parameter for characterizing diffusion.
  • Extracting MSD from FCS data is common but not always accurate.

Purpose of the Study:

  • To determine the conditions under which MSD can be reliably obtained from FCS.
  • To investigate the accuracy of apparent MSD derived from FCS for single polymer chain diffusion.
  • To propose a method for validating the reliability of FCS-derived MSD.

Main Methods:

  • Utilizing computer simulations to model polymer chain diffusion.
  • Calculating both true MSD and apparent MSD from simulated FCS data.
  • Analyzing the deviations between true and apparent MSD under various approximations.

Main Results:

  • The procedure for retrieving MSD from FCS is not universally valid.
  • Apparent MSD obtained from FCS systematically deviates from the true MSD for polymer diffusion.
  • Simulation results highlight the limitations of standard FCS analysis for complex diffusion.

Conclusions:

  • The reliability of apparent MSD from FCS requires careful consideration of experimental conditions.
  • FCS-derived MSD can be systematically inaccurate, particularly for polymer dynamics.
  • Measurements across varying focal spot sizes offer a method to verify the accuracy of apparent MSD.