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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Viral Recombination00:57

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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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Viral Mutations00:36

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Novel Sequence Discovery by Subtractive Genomics
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Novel Sequence Discovery by Subtractive Genomics

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A disruptive sequencer meets disruptive publishing.

Nick Loman1, Sarah Goodwin2, Hans Jansen3

  • 1Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.

F1000Research
|March 22, 2016
PubMed
Summary
This summary is machine-generated.

The Oxford Nanopore MinION offers long-read sequencing from single DNA molecules. A new F1000Research channel will track its rapid evolution and analysis methods.

Keywords:
MinIONMinION access programmeMinION analysis consortiumOxford nanopore technologynanoporesequencing

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Area of Science:

  • Genomics and Bioinformatics
  • Molecular Biology

Background:

  • The Oxford Nanopore MinION provides a portable platform for DNA sequencing.
  • It is known for generating exceptionally long sequence reads from single DNA molecules.
  • The technology is rapidly advancing with frequent updates to hardware and chemistry.

Purpose of the Study:

  • To establish a dedicated channel on F1000Research for nanopore sequencing.
  • To facilitate open discussion and dissemination of new analysis methods for nanopore data.
  • To keep the scientific community updated on the MinION platform's rapid evolution.

Main Methods:

  • The study introduces a new F1000Research channel.
  • This channel will serve as a repository for research and discussion on nanopore sequencing.
  • It aims to aggregate updates and analytical approaches for the MinION platform.

Main Results:

  • A new F1000Research channel has been launched.
  • This platform will centralize information on nanopore sequencing advancements.
  • It provides a venue for the scientific community to share and discuss new analytical techniques.

Conclusions:

  • The rapid evolution of nanopore sequencing necessitates dedicated platforms for knowledge sharing.
  • The F1000Research channel will support the growing field of nanopore data analysis.
  • This initiative aims to accelerate the adoption and effective utilization of MinION technology.