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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Mar 23, 2026

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Liver Cell Culture Devices.

B Andria¹, A Bracco², G Cirino³

¹Center of Biotechnologies, Cardarelli Hospital, Naples, Italy; †Faculty of Pharmacy, 'Federico II" University, Naples, Italy.

|March 22, 2016

Summary

This summary is machine-generated.

New artificial liver cell culture systems aid drug development and disease prediction. These advanced devices improve the viability and function of liver cells for better in vitro screening and personalized medicine insights.

Keywords:

Bioreactor Cell culture devices Drug delivery Human liver cells

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Last Updated: Mar 23, 2026

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

Author Spotlight: Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications

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Published on: October 20, 2023

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

Published on: November 22, 2024

Area of Science:

Biotechnology and Bioengineering
Hepatology and Drug Metabolism
In Vitro Toxicology

Background:

Liver cell culture systems are crucial for drug development and understanding liver diseases.
Numerous artificial liver cell culture devices have been developed over the last 15 years.
These systems serve applications ranging from temporary organ replacement to in vitro drug screening.

Purpose of the Study:

To review and summarize artificial human liver cell culture systems developed between 2003 and 2009.
To categorize existing liver cell culture devices based on their configuration (2D and 3D).
To discuss essential features for optimal liver cell culture and future system development.

Main Methods:

Literature review of artificial human liver cell culture systems using PubMed (2003-2009).
Classification of devices into 2D (e.g., monolayer, sandwich) and 3D (e.g., slices, spheroids, bioreactors) configurations.
Discussion of key components: scaffolds, oxygenation, extracellular matrix, co-cultures, and shear stress.

Main Results:

A variety of 2D and 3D liver cell culture systems exist, each with specific advantages.
Essential factors influencing liver cell viability and function in culture include scaffold type, oxygenation, matrix, cell co-culture, and shear stress.
Miniaturization and high-throughput systems are emerging trends.

Conclusions:

Several effective liver cell culture systems are available for predicting general population hepatotoxicity and drug metabolism.
The choice of system depends on the specific application and desired outcome.
Genomic analysis may be necessary for predicting individual hepatotoxicity.