Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
MicroRNAs01:22

MicroRNAs

12.0K
12.0K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.8K
Experimental RNAi02:15

Experimental RNAi

8.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ribozyme-Mediated Knockdown of lncRNA Gene Expression in <i>Drosophila</i>.

Bio-protocol·2025
Same author

Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in <i>Drosophila</i>.

eLife·2024
Same author

Robust and heritable knockdown of gene expression using a self-cleaving ribozyme in Drosophila.

Genetics·2024
Same author

Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in <i>Drosophila</i>.

bioRxiv : the preprint server for biology·2024
Same author

Energy metabolism modulates the regulatory impact of activators on gene expression.

Development (Cambridge, England)·2023
Same author

Energy metabolism modulates the regulatory impact of activators on gene expression.

bioRxiv : the preprint server for biology·2023

Related Experiment Video

Updated: Mar 23, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.8K

MicroRNA function in Drosophila melanogaster.

Richard W Carthew1, Pamela Agbu2, Ritika Giri2

  • 1Department of Molecular Biosciences, Northwestern University Evanston, IL 60208, USA; Department of Biochemistry and Molecular Genetics, Northwestern University, Chicago, IL 60611, USA; Robert H. Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA.

Seminars in Cell & Developmental Biology
|March 23, 2016
PubMed
Summary

MicroRNAs are key regulators in fruit fly biology, influencing development and robustness. Their essential roles in development and robustness are revealed under varying environmental or genetic conditions.

Keywords:
DrosophilaMiRNAsMicroRNAs

More Related Videos

Maintenance of a Drosophila melanogaster Population Cage
08:03

Maintenance of a Drosophila melanogaster Population Cage

Published on: March 15, 2016

14.2K
RNAi Interference by dsRNA Injection into Drosophila Embryos
08:30

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

16.9K

Related Experiment Videos

Last Updated: Mar 23, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.8K
Maintenance of a Drosophila melanogaster Population Cage
08:03

Maintenance of a Drosophila melanogaster Population Cage

Published on: March 15, 2016

14.2K
RNAi Interference by dsRNA Injection into Drosophila Embryos
08:30

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

16.9K

Area of Science:

  • * Molecular Biology
  • * Developmental Biology
  • * Genetics

Background:

  • * MicroRNAs (miRNAs) are crucial regulators of gene expression in animal genomes.
  • * Their roles in various biological processes are increasingly recognized.
  • * The fruit fly, Drosophila melanogaster, serves as a model organism for studying fundamental biological mechanisms.

Purpose of the Study:

  • * To review the multifaceted roles of microRNAs in Drosophila melanogaster.
  • * To highlight miRNA involvement in tissue growth, germ cell development, and neurobiology.
  • * To explore the contribution of microRNAs to biological robustness.

Main Methods:

  • * Comprehensive literature review of studies on microRNAs in Drosophila.
  • * Analysis of experimental data linking miRNAs to developmental processes.
  • * Examination of theoretical frameworks for miRNA-mediated robustness.

Main Results:

  • * MicroRNAs significantly impact tissue growth, germ cell development, and central nervous system function in flies.
  • * Specific miRNAs are implicated in hormone action and developmental pathways.
  • * MicroRNA-mediated regulation contributes to the robustness of gene regulatory networks.

Conclusions:

  • * MicroRNAs are essential for normal development and physiological functions in Drosophila.
  • * The robust nature of miRNA functions becomes apparent under conditions of environmental or genetic variation.
  • * Understanding miRNA roles provides insights into developmental plasticity and evolutionary adaptation.