Abscisic acid transcriptomic signaling varies with grapevine organ
Supakan Rattanakon1, Ryan Ghan1, Gregory A Gambetta2
1Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, 89557, USA.
BMC Plant Biology
|March 23, 2016
Summary
Abscisic acid (ABA) triggers unique gene expression changes in different grapevine organs within hours. Understanding these organ-specific ABA responses is key to comprehending plant hormone action and water deficit tolerance.
Area of Science:
- Plant Biology
- Molecular Biology
- Genomics
Background:
- Abscisic acid (ABA) is a crucial plant hormone regulating development and stress responses.
- Early transcriptional responses to ABA are vital for understanding plant adaptation.
- Grapevine (Vitis vinifera) is an economically important crop susceptible to environmental stresses.
Purpose of the Study:
- To investigate the organ-specific transcriptional effects of ABA in grapevine.
- To identify early molecular responses to ABA treatment across different grapevine organs.
- To elucidate how ABA signaling pathways differ across grapevine tissues.
Main Methods:
- Treatment of grapevine organs (berries, shoot tips, leaves, roots, cell cultures) with 10 μM (S)-(+)-ABA for 2 hours.
- Whole genome microarray analysis using NimbleGen Vitis vinifera arrays.
- Bioinformatic analysis including Cytoscape and BiNGO for pathway identification.
Main Results:
- 839 genes showed significant organ-specific transcript abundance changes in response to ABA.
- No single gene responded identically across all organs.
- Affected pathways include flavonoid metabolism, ROS, light, and temperature responses; ion/water transport (roots); and protein phosphorylation (shoot tips, roots).
- ABA modulated genes involved in its own metabolism (CYP707As, UGTs, PP2Cs) and transcription factors (AP2/ERF, MYC/MYB, bZIP/AREB).
- Roots exhibited the highest number of differentially expressed genes, followed by cell cultures, leaves, berries, and shoot tips.
Conclusions:
- Grapevine organ responses to short-term ABA treatment are unique.
- ABA signaling pathways exhibit significant organ-specificity.
- Understanding organ-specific ABA responses is critical for comprehending hormone action and plant adaptation to water deficit.
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