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Published on: November 10, 2015
Analyzing Deoxyribose Nucleic Acid from Malaria Rapid Diagnostic Tests to Study Plasmodium falciparum Genetic
Cécile Nabet1, Safiatou Doumbo1, Fakhri Jeddi1
1UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy.
Abstract:
We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity of Plasmodium falciparum in Mali. Genetic diversity was assessed via multiple loci variable number of tandem repeats analysis (MLVA). We performed DNA extraction from 104 positive and 30 negative used mRDTs that had been stored at ambient temperature for up to 14 months. Extracted DNA was analyzed via quantitative polymerase chain reaction (qPCR), and MLVA genotyping was then assessed on positive qPCR samples. Eighty-three of the positive mRDTs (83/104, 79.8%) and none of the negative mRDTs were confirmed P. falciparum positive via qPCR. We achieved complete genotyping of 90.4% (75/83) of the qPCR-positive samples. Genotyping revealed high genetic diversity among P. falciparum populations in Mali and an absence of population clustering. We show that mRDTs are useful to monitor P. falciparum genetic diversity and thereby can provide essential data to guide malaria control programs.
