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Updated: Mar 23, 2026

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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
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Partial nitrification in MBBRs for mainstream deammonification with thin biofilms and alternating feed supply
M Piculell1, M Christensson1, K Jönsson2
1AnoxKaldnes AB, Klosterängsvägen 11A, Lund SE-226 47, Sweden
Summary
This study explores a novel nitrogen removal method using nitritation and anammox in a two-stage reactor. The process achieved high nitrite accumulation and efficient ammonia and total nitrogen removal rates.
Area of Science:
- Environmental Engineering
- Microbiology
- Wastewater Treatment
Background:
- Conventional nitrogen removal processes are energy-intensive.
- Developing efficient and cost-effective nitrogen removal technologies is crucial for environmental protection.
- Nitritation-anammox offers a promising alternative for mainstream nitrogen removal.
Purpose of the Study:
- To investigate a new principle for mainstream nitrogen removal using nitritation followed by anammox.
- To optimize a two-stage moving bed biofilm reactor (MBBR) for this process.
- To evaluate the efficiency of ammonia and nitrogen removal under specific operational conditions.
Main Methods:
- Utilized a two-stage moving bed biofilm reactor (MBBR) configuration.
- Optimized the first stage for nitritation using thin biofilms (<200 μm) and alternating feed conditions (wastewater at 15°C, reject water at 30°C).
- Optimized the second stage for anammox using the effluent from the nitritation stage.
Main Results:
- Achieved successful nitritation with a nitrite accumulation ratio consistently above 75%.
- Observed significant ammonia removal rates ranging from 0.25 to 0.50 g NH4-N/L·d.
- Attained nitrogen removal rates exceeding 0.20 g TN/L·d in the anoxic second stage.
Conclusions:
- The two-stage MBBR configuration effectively facilitates mainstream nitrogen removal via nitritation and anammox.
- Alternating feed conditions and controlled biofilm thickness are key to optimizing nitritation.
- The studied process demonstrates high efficiency for both ammonia and overall nitrogen removal.
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