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Related Concept Videos

The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Replication in Eukaryotes01:29

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
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Updated: Mar 23, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

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The Eukaryotic Replisome Goes Under the Microscope.

Mike O'Donnell1, Huilin Li2

  • 1DNA Replication Lab, The Rockefeller University, 1230 York Avenue, New York, New York, USA; Howard Hughes Medical Institute.

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|March 23, 2016
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Summary
This summary is machine-generated.

Recent structural studies reveal the eukaryotic replisome

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Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The eukaryotic replication fork is a complex molecular machine responsible for DNA duplication.
  • Understanding its structure is crucial for comprehending DNA replication fidelity and regulation.
  • Recent advancements in structural biology have provided unprecedented insights into its components.

Purpose of the Study:

  • To summarize recent structural advances in the eukaryotic replisome.
  • To highlight novel findings regarding the architecture and function of the replisome.

Main Methods:

  • Electron microscopy
  • X-ray crystallography

Main Results:

  • New structures of key replisome components, including the CMG helicase and DNA polymerases.
  • The first structural view of a complete eukaryotic core replisome comprising 20 proteins.
  • An unanticipated architecture of the core replisome with polymerases positioned above and below the helicase.
  • Structures revealing the Mcm2 complex interacting with nucleosomes (H3/H4 tetramer).

Conclusions:

  • Recent structural data have unveiled a complex and unanticipated architecture of the eukaryotic replisome.
  • These findings suggest a direct role for the replisome in nucleosome handling, impacting DNA organization and gene regulation.