Related Experiment Video
Updated: Mar 23, 2026
07:31
Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
Published on: February 17, 2023
1.7K
Precise Editing of the Zebrafish Genome Made Simple and Efficient
Kazuyuki Hoshijima1, Michael J Jurynec1, David Jonah Grunwald1
1Department of Human Genetics, University of Utah, Salt Lake City, UT 84112, USA.
Developmental Cell
|March 23, 2016
Summary
Researchers developed efficient methods for zebrafish genome editing using homologous recombination and double-strand breaks. These techniques enable precise genetic modifications, improving the study of biological processes and diseases.
Area of Science:
- Genetics
- Molecular Biology
- Zebrafish Models
Background:
- Zebrafish are valuable models for biological research and disease modeling.
- Efficient and heritable genome editing is crucial for advancing zebrafish-based studies.
Purpose of the Study:
- To develop simple and efficient methods for creating heritable modifications in the zebrafish genome.
- To enhance the recovery and applicability of genome editing events in zebrafish.
Main Methods:
- Utilizing homologous recombination between host genome and dsDNA donor molecules.
- Inducing chromosomally targeted double-strand breaks to stimulate editing.
- Employing reporter genes for improved recovery of edited alleles and conditional mutations.
Main Results:
- Demonstrated successful generation of various heritable genome modifications.
- Created alleles with single codon changes, epitope-tagged proteins, reporter proteins, and conditional mutations.
- Showcased the ability to replace several kilobase-long tracts of genome sequence.
Conclusions:
- The developed methods are practicable for a broad range of genome editing events in zebrafish.
- These advancements significantly enhance the utility of zebrafish for studying biological processes and modeling diseases.
