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Biodegradation of Phenol by Bacteria Strain Acinetobacter Calcoaceticus PA Isolated from Phenolic Wastewater
Zhenghui Liu1,2, Wenyu Xie3,4, Dehao Li5,6
1Department of Environmental Engineering, School of Environmental and Biological Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China. lzhhok@126.com.
Abstract:
A phenol-degrading bacterium strain PA was successfully isolated from the effluent of petrochemical wastewater. Based on its morphological, physiological and biochemical characteristics, the strain PA was characterized as a Gram-negative, strictly aerobic, nonmotile and short rod-shaped bacterium that utilizes phenol as a sole carbon and energy source. 16S rDNA sequence analysis revealed that this strain is affiliated to Acinetobacter calcoaceticus in the group of Gammaproteobacteria. The strain was efficient in removing 91.6% of the initial 800 mg ∙ L(-1) phenol within 48 h, and had a tolerance of phenol concentration as high as 1700 mg ∙ L(-1). These results indicated that A. calcoaceticus possesses a promising potential in treating phenolic wastewater.
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