Related Experiment Video
Updated: Mar 23, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Simultaneous saccharification and fermentation (SSF) of jackfruit seed powder (JFSP) to l-lactic acid and to
Nimisha Rajendran Nair1, K Madhavan Nampoothiri1, Rintu Banarjee2
1Biotechnology Division, National Institute for Interdisciplinary Science and Technology, CSIR, Trivandrum 695019, India.
Abstract:
A newly isolated amylolytic lactic acid bacterium, Streptococcus equinus, was used for the production of l-lactic acid from jackfruit seed powder (JFSP) by simultaneous saccharification and fermentation (SSF). After optimization of shake flask fermentation by a response surface box-behnken design, the maximum lactate titer was 109g/L from 200g/L jackfruit seed powder. Amberlite IRA67, a weak base resin, was used to recover pure lactic acid from fermented broth and subsequently used for the synthesis of polylactic acid by direct condensation polymerization method with a yield of 62%.
Related Concept Videos
Production of Organic Acids
Microbes in the Production of Fermented Foods
Biosynthesis of Polysaccharides
Microbes in Food Production
Bioplastics
Microbial Fermentation

