Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.8K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.2K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.2K
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

11.4K
Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
11.4K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

113.2K
Overview
113.2K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

5.1K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
5.1K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

3.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the seven critical residues that control ZIKV-DENV cross-reactivity to engineer a non-cross-reactive ZIKV vaccine.

Cell reports·2025
Same author

Identification of alkynyl nicotinamide HSN748 as a RET solvent-front mutant inhibitor with intracranial efficacy.

RSC medicinal chemistry·2025
Same author

Correction for Ramos et al., "Broadly protective bispecific antibodies that simultaneously target influenza virus hemagglutinin and neuraminidase".

mBio·2025
Same author

Engineered antibodies targeted to bacterial surface integrate effector functions with toxin neutralization to provide superior efficacy against bacterial infections.

medRxiv : the preprint server for health sciences·2024
Same author

Alkynyl nicotinamides show antileukemic activity in drug-resistant acute myeloid leukemia.

The Journal of clinical investigation·2024
Same author

Broadly protective bispecific antibodies that simultaneously target influenza virus hemagglutinin and neuraminidase.

mBio·2024

Related Experiment Video

Updated: Mar 23, 2026

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
10:11

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

37.1K

Chasing Ebola through the Endosomal Labyrinth.

M Javad Aman1

  • 1Integrated BioTherapeutics, Inc., Gaithersburg, Maryland, USA javad@integratedbiotherapeutics.com.

Mbio
|March 24, 2016
PubMed
Summary

A new live-cell imaging method tracks Ebola virus (EBOV) entry in real time. This study reveals fusion occurs in late endosomes and is inhibited by neutralizing antibodies like KZ52 and ZMapp.

Area of Science:

  • Virology
  • Cell Biology
  • Biochemistry

Background:

  • Ebola virus (EBOV) entry involves complex glycoprotein transformations within endosomes.
  • Previous methods for studying EBOV entry were limited to static or indirect analyses.
  • A novel live-cell imaging technique offers real-time insights into EBOV entry dynamics.

Purpose of the Study:

  • To develop and validate a live-cell imaging assay for real-time tracking of EBOV entry.
  • To elucidate the precise location and timing of EBOV fusion events within the endosomal pathway.
  • To investigate the mechanism of action of EBOV-neutralizing antibodies.

Main Methods:

  • Development of a novel live-cell imaging assay for real-time observation of EBOV entry.
  • Utilizing the assay to track the transformational journey of EBOV glycoproteins.

More Related Videos

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.7K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.8K

Related Experiment Videos

Last Updated: Mar 23, 2026

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
10:11

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

37.1K
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

1.7K
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.8K
  • Analyzing fusion events in relation to endosomal maturation and Niemann-Pick C1 (NPC1) presence.
  • Investigating the impact of neutralizing antibodies (e.g., KZ52, ZMapp) on fusion.

    • Main Results:

    • EBOV fusion initiates in maturing early endosomes and completes in late endosomes, exclusively within Niemann-Pick C1 (NPC1)-positive compartments.
    • Lipid mixing and productive fusion are temporally separated, with a protease-dependent step in between.
    • Neutralizing antibodies, including KZ52 and ZMapp, directly inhibit EBOV membrane fusion.

    Conclusions:

    • The novel live-cell imaging method provides unprecedented real-time data on EBOV entry mechanisms.
    • EBOV fusion is a complex, spatially and temporally regulated process occurring in specific endosomal compartments.
    • Neutralizing antibodies target the membrane fusion step, offering a potential therapeutic strategy against EBOV infection.