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Updated: Mar 23, 2026
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
Preparation of nanoliposome loaded with peanut peptide fraction: stability and bioavailability
Kui-Jie Gong1, Ai-Min Shi2, Hong-Zhi Liu2
1Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China. wangqiang06@caas.cn and Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Abstract:
Nanoliposome loaded with peanut peptide fraction (PPF) prepared by high pressure microfluidization (HPM) treatment was investigated as well as its stability and bioavailability. PPF showed hydrophilicity character with a solubility of 97.50 ± 2.31 mg mL(-1) in aqueous solution. HPM treatment can prepare nanoliposome but decreased encapsulation efficiency (EE). A pressure of 120 MPa was the appropriate parameter where the particle size and EE of nanolipsome was 79.67 ± 1.85 nm and 65.12 ± 2.96%, respectively. Crude liposome and nanoliposome both showed good stability under different pH conditions, even at pH value of 2.0. Nanoliposome behaved better in vitro controlled release than crude liposome. Most important of all, nanoliposome had the highest angiotension converting enzyme (ACE) inhibitory activity after simulated gastrointestinal tract (GIT) digestion. Morphology of digested liposome proved that nanoliposome can keep relative integrity in structure although it suffered a lot of attack.
