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Related Concept Videos

Voltammetric Techniques: Cyclic Voltammetry01:10

Voltammetric Techniques: Cyclic Voltammetry

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Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
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Voltammetric Techniques: Pulse Voltammetry01:17

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Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
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Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

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Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
831
Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

827
Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
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Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

1.5K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
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Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

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In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
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Updated: Mar 23, 2026

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
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Fast Selective Detection of Pyocyanin Using Cyclic Voltammetry.

Fatima AlZahra'a Alatraktchi1,2,3, Sandra Breum Andersen4, Helle Krogh Johansen5,6

  • 1Department of Micro- and Nanotechnology, Technical University of Denmark, Ørsteds Plads, 2800 Kgs. Lyngby, Denmark. faaat@nanotech.dtu.dk.

Sensors (Basel, Switzerland)
|March 24, 2016
PubMed
Summary

A new electrochemical method enables the selective detection of pyocyanin, a key indicator of Pseudomonas aeruginosa infections. This inexpensive technique offers real-time monitoring in clinical settings, aiding patient diagnosis and treatment.

Keywords:
cyclic voltammetrydiagnosiselectrochemical detectionpyocyaninquorum sensing

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Area of Science:

  • Electrochemistry
  • Analytical Chemistry
  • Microbiology

Background:

  • Pyocyanin is a unique virulence factor produced by Pseudomonas aeruginosa.
  • Accurate detection of pyocyanin is crucial for identifying P. aeruginosa infections in patients.
  • Existing detection methods face limitations due to interfering redox-active compounds in biological samples.

Purpose of the Study:

  • To develop a direct and selective electrochemical method for pyocyanin detection.
  • To quantify pyocyanin in complex electroactive environments using commercially available electrodes.
  • To establish a cost-effective and rapid diagnostic tool for P. aeruginosa infections.

Main Methods:

  • Utilized cyclic voltammetry with commercially available electrodes.
  • Investigated a potential detection window for pyocyanin between -1.0 V and 1.0 V.
  • Validated the method using human saliva samples spiked with known pyocyanin concentrations.

Main Results:

  • Identified a selective pyocyanin detection window of 0.58–0.82 V, unaffected by interferents.
  • Achieved linear quantification of pyocyanin (R² = 0.991) in the clinically relevant range of 2–100 µM.
  • Demonstrated high accuracy in human saliva samples with a standard deviation of 2.5% ± 1%.

Conclusions:

  • The developed electrochemical method provides selective and accurate pyocyanin quantification.
  • This inexpensive and rapid technique is suitable for real-time monitoring of P. aeruginosa infections.
  • The method holds potential for clinical application in diagnosing and managing Pseudomonas aeruginosa infections.