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Related Experiment Video

Updated: Mar 23, 2026

Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster
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Rescuing axons from degeneration does not affect retinal ganglion cell death.

S de Lima1, B S Mietto2, C Paula1

  • 1Laboratório de Neurobiologia da Retina, Centro de Ciências da Saúde, Instituto de Biofísica Carlos Chagas Filho, Rio de Janeiro, RJ, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas
|March 24, 2016
PubMed
Summary
This summary is machine-generated.

Calpain inhibitors delayed axonal degeneration after optic nerve injury in rats. However, this treatment did not improve the survival of retinal ganglion cells (RGCs).

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Regenerative Medicine

Background:

  • Traumatic central nervous system injury triggers Wallerian degeneration (WD) in axons.
  • This degeneration involves cytoskeleton breakdown and inhibitory protein expression, hindering axonal regrowth.
  • Increased intracellular calcium activates calpains, proteases that degrade cytoskeletal proteins, initiating WD.

Purpose of the Study:

  • To investigate if inhibiting calpains impacts retinal ganglion cell (RGC) survival after optic nerve crush.
  • To determine if preventing axonal degeneration affects RGC survival.

Main Methods:

  • Male Wistar rats underwent optic nerve crush.
  • An exogenous calpain inhibitor was directly applied using Evlax copolymer resin.
  • Axonal degeneration and RGC survival were assessed 4 days post-injury.

Main Results:

  • Calpain inhibitor treatment delayed the onset of Wallerian degeneration.
  • A significant decrease in degenerated fibers and increase in preserved fibers were observed.
  • Preserved fibers maintained a normal G-ratio, indicating structural integrity.

Conclusions:

  • Calpain inhibition effectively prevented axonal degeneration in the optic nerve.
  • Despite preventing axonal damage, calpain inhibition did not enhance RGC survival after optic nerve injury.