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Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens
Irina V Safenkova1, Galina K Pankratova1, Ilya A Zaitsev2
1A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia.
Analytical and Bioanalytical Chemistry
|March 24, 2016
Summary
A new Multiarray on a Test Strip (MATS) assay rapidly detects eight potato pathogens simultaneously. This method combines immunochromatography and array techniques for efficient on-site disease control.
Area of Science:
- Plant Pathology
- Biotechnology
- Immunodiagnostics
Background:
- Potato pathogens pose a significant threat to crop yield and quality.
- Existing diagnostic methods can be time-consuming and require specialized laboratory equipment.
Purpose of the Study:
- To develop a rapid, high-throughput assay for the simultaneous detection of eight key potato pathogens.
- To validate the assay's performance using potato leaf extracts for on-site application.
Main Methods:
- Development of a Multiarray on a Test Strip (MATS) assay.
- Utilizing immunochromatography with specific antibodies immobilized on a test strip.
- Visual detection of colored immune complexes for pathogen identification.
Main Results:
- Simultaneous detection of eight priority potato pathogens with varying limits of detection (1-300 ng/mL for viruses, 10^4 cells/mL for bacteria).
- Analysis time of 15 minutes.
- Sensitivity comparable to traditional Enzyme-Linked Immunosorbent Assay (ELISA).
Conclusions:
- The MATS assay offers a rapid and efficient tool for simultaneous potato pathogen detection.
- The technique is suitable for on-site diagnostics, enabling timely disease management.
- Confirmed 100% efficiency using commercial Lateral Flow Immunoassay (LFIA) test strips on potato leaf extracts.
