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Gene expression pattern of glucose transporters in the skeletal muscles of newly hatched chicks

Saki Shimamoto1, Daichi Ijiri1, Mana Kawaguchi1

  • 1a Department of Biochemical Science and Technology , Kagoshima University , Kagoshima , Japan.

Bioscience, Biotechnology, and Biochemistry
|March 24, 2016
PubMed

Insights

Newly hatched chicks show higher glucose transporter 1 (GLUT1) mRNA in chest muscles. This suggests a link between AKT signaling and GLUT1 expression in avian muscle development.

Area of Science:

  • Animal Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Glucose transporters (GLUTs) are crucial for cellular glucose uptake.
  • Understanding GLUT expression patterns is vital for muscle metabolism research.
  • The role of specific GLUTs in avian muscle development requires further investigation.

Purpose of the Study:

  • To investigate the gene expression of GLUT1, GLUT3, GLUT8, and GLUT12 in specific muscles of newly hatched chicks.
  • To explore the relationship between AKT signaling and GLUT1 expression in avian skeletal muscle.

Main Methods:

  • Quantitative analysis of GLUT1, GLUT3, GLUT8, and GLUT12 mRNA levels using gene expression profiling.
  • Measurement of phosphorylated AKT protein levels via Western blotting.
  • Comparative analysis of gene and protein expression across pectoralis major, pectoralis minor, biceps femoris, and sartorius muscles.

Main Results:

  • GLUT1 mRNA expression was significantly higher in the pectoralis major muscle compared to other examined muscles.
  • Elevated levels of phosphorylated AKT were observed in the pectoralis major muscle.
  • A potential correlation between high GLUT1 mRNA levels and increased phosphorylated AKT was identified.

Conclusions:

  • The pectoralis major muscle exhibits a distinct glucose transporter expression profile in newly hatched chicks.
  • The findings suggest a regulatory role for AKT signaling in modulating GLUT1 expression in avian skeletal muscle.
  • Further research is warranted to elucidate the precise mechanisms underlying AKT-mediated GLUT1 regulation in muscle tissue.

Keywords:
AKTchickenglucose transporterinsulin receptorskeletal muscle

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