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Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application
V Boonsaeng1, K Chansiri, P Vilasineekul
1Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
The Southeast Asian Journal of Tropical Medicine and Public Health
|December 1, 1989
Abstract:
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.