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Published on: October 20, 2023
Identification and Biological Activities of Long-Chain Peptaibols Produced by a Marine-Derived Strain of Trichoderma
Mustapha Mohamed-Benkada1, Yves François Pouchus2, Philippe Vérité3
1Département de Biotechnologie, Faculté des Sciences de la Nature et de la Vie, Université des Sciences et de la Technologie d'Oran-Mohamed Boudiaf (USTO-MB), El Mnaouar, B.P. 1505, Bir El Djir, 31000, Oran, Algeria. professionnelle@hotmail.ca.
Abstract:
Six long-chain peptaibols, 1 - 6, were identified from agar cultures of a marine-derived Trichoderma longibrachiatum Rifai strain (MMS151) isolated from blue mussels. The structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (ESI-IT-MS) and GC/EI-MS. The long-chain peptaibols exhibited the general building scheme Ac-Aib-Ala-Aib-Ala-Aib-XXX-Gln-Aib-Vxx-Aib-Gly-XXX-Aib-Pro-Vxx-Aib-XXX-Gln-Gln-Pheol and were similar or identical to recurrent 20-residue peptaibols produced by Trichoderma spp. Three new sequences were identified and were called longibrachins A-0, A-II-a, and A-IV-b. The isolated peptaibols were assayed for cytotoxic, antibacterial, and antifungal activities, and acute toxicity on Dipteran larvae.
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