Towards "bionic" proteins: replacement of continuous sequences from HIF-1α with proteomimetics to create functional
George M Burslem1, Hannah F Kyle2, Alexander L Breeze3
1School of Chemistry, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK and Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane, Leeds, LS29JT, UK. a.j.wilson@leeds.ac.uk.
Abstract:
Using the HIF-1α transcription factor as a model, this manuscript illustrates how an extended sequence of α-amino acids in a polypeptide can be replaced with a non-natural topographical mimic of an α-helix comprised from an aromatic oligoamide. The resultant hybrid is capable of reproducing the molecular recognition profile of the p300 binding sequence of HIF-1α from which it is derived.


