Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.5K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.7K
2.7K
Transcription Factors02:16

Transcription Factors

83.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
83.8K
Transcription Factors02:16

Transcription Factors

26.6K
26.6K
General Transcription Factors01:30

General Transcription Factors

7.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.5K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.8K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TGF-β controls developmental fate and functional identity of thymic γδ T cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

A Universal Preparation Strategy for Carbon-Supported Metal Catalysts with Excellent Hydrogenation Performance: Ligand Regulation-Assisted Solution Carbonization.

Inorganic chemistry·2026
Same author

Probiotic Properties of Pediococcus acidilactici Z123 and its Intervention Effects and Mechanisms on Hypercholesterolemia in Mice.

Probiotics and antimicrobial proteins·2026
Same author

In-Plane Conductivity as a Descriptor of Apparent Durability of RuO<sub>2</sub> Anodes in PEM Water Electrolysis.

Nano letters·2026
Same author

PIP<sub>2</sub> activation of the cardiac I<sub>Ks</sub> potassium channel.

Nature communications·2026
Same author

Green tea catechin EGCG attenuates hippocampal atrophy and cognitive impairment in obesity via autophagy signaling.

NPJ science of food·2026

Related Experiment Video

Updated: Mar 23, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

8.6K

SM-TF: A structural database of small molecule-transcription factor complexes.

Xianjin Xu1, Zhiwei Ma1,2, Hongmin Sun3

  • 1Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri, 65211.

Journal of Computational Chemistry
|March 25, 2016
PubMed
Summary

A new database of small molecule-transcription factor (SM-TF) complexes aids drug design. It provides 3D structures for 176 transcription factors, supporting the development of new therapies targeting gene expression.

Keywords:
drug designgene regulationstructural databasetherapeutic targettranscription factor

More Related Videos

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.3K
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.9K

Related Experiment Videos

Last Updated: Mar 23, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

8.6K
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.3K
Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

2.9K

Area of Science:

  • Biochemistry and Molecular Biology
  • Structural Biology
  • Pharmacology

Background:

  • Transcription factors (TFs) regulate gene expression, and their dysfunction is linked to numerous diseases.
  • Over 30 TFs are therapeutic targets for approximately 9% of approved drugs, highlighting their importance in medicine.

Purpose of the Study:

  • To create a comprehensive structural database of small molecule-transcription factor (SM-TF) complexes.
  • To provide a valuable resource for structure-based drug design targeting TFs.

Main Methods:

  • Development of an online database (http://zoulab.dalton.missouri.edu/SM-TF) for SM-TF complexes.
  • Inclusion of 3D structures of small molecules and their binding sites on TFs.
  • Linking entries to UniProt, sequence-based TF databases, and DrugBank for human TF-drug information.

Main Results:

  • The SM-TF database currently contains 934 entries, covering 176 TFs across various species.
  • The database is organized by species and organism.
  • Provides links to relevant external databases for comprehensive information.

Conclusions:

  • The SM-TF database is a significant resource for researchers and drug designers.
  • Facilitates structure-based drug discovery for TF-related diseases.
  • Enhances understanding of small molecule interactions with transcription factors.