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Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug
Published on: May 17, 2024
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Antihypertensive peptides from curd
Melani Chathurika Dabarera1, Lohini V Athiththan1, Rasika P Perera1
1Department of Biochemistry, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka.
Ayu
|March 25, 2016
Summary
Curd peptides effectively inhibit angiotensin-converting enzyme (ACE), with activity significantly increasing after enzymatic digestion. This study identified potent antihypertensive peptides in commercial curd brands, highlighting their potential health benefits.
Area of Science:
- Biochemistry
- Food Science
- Pharmacology
Background:
- Curd peptides are known to possess antihypertensive properties by inhibiting angiotensin-converting enzyme (ACE).
- The composition and bioactivity of these peptides can differ based on the bacterial species and milk source used in fermentation.
- This study focuses on commercial curd brands available in Sri Lanka.
Purpose of the Study:
- To isolate and quantify antihypertensive peptides in two Sri Lankan curd brands.
- To evaluate the angiotensin-converting enzyme (ACE) inhibitory activity of these peptides before and after enzymatic digestion.
Main Methods:
- Whey peptides were isolated using reverse-phase-high-performance liquid chromatography (HPLC).
- ACE inhibitory activity was determined using the modified Cushman and Cheung method.
- Curd samples underwent enzymatic digestion with pepsin, trypsin, and carboxypeptidase-A.
Main Results:
- HPLC analysis revealed similar elution patterns for whey peptides from both brands.
- Brand 2 exhibited a higher concentration of penta-octapeptides, with a pentapeptide showing 90% ACE inhibition.
- Post-digestion, di- and tri-peptides demonstrated enhanced ACE inhibitory activity, reaching up to 96% inhibition.
Conclusions:
- Curd contains bioactive peptides with significant ACE inhibitory potential.
- Enzymatic digestion further enhances the antihypertensive activity of these peptides.
- Commercial curd brands possess peptides that could be beneficial for managing hypertension.
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