Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

830
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
830
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.6K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.6K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

653
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
653
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

7.2K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
7.2K
Southern Blot02:57

Southern Blot

24.4K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
24.4K
DNA Isolation01:24

DNA Isolation

46.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
46.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SARS-CoV-2 Persistence and Cardiovascular Sequelae in the Post-COVID Era: A Public Health Microbiology Perspective on Sudden Cardiac Death and Pulmonary Thromboembolism.

Microorganisms·2026
Same author

Eating Habits, Body Weight Perception, and Psycho-Emotional Factors Among Romanian University Students: A Cross-Sectional Study.

Nutrients·2026
Same author

Perioperative Anemia, Transfusion Practices, and Patient Blood Management: Lessons from the COVID-19 Pandemic.

Hematology reports·2026
Same author

Predicting Hungry Bone Syndrome with Interpretable Machine Learning: A Single-Center Cohort of Dialysis Patients Undergoing Parathyroidectomy.

Diagnostics (Basel, Switzerland)·2026
Same author

BPC-157 as an Investigational Peptide Therapeutic: Biopharmaceutical Challenges, Formulation Strategies, and Translational Development Barriers.

Pharmaceutics·2026
Same author

Diet-Microbiome-Brain Axis and Mental Health: Biological Mechanisms and Nutritional Implications.

Nutrients·2026
Same journal

Profilin-1 as a Potential Biomarker in Acute Ischemic Stroke: Predictive Value for Large Vessel Occlusion and Mechanical Thrombectomy.

Clinical laboratory·2026
Same journal

Soluble Thrombomodulin can Predict In-Hospital Mortality of Community-Acquired Pneumonia Patients in Intensive Care Unit.

Clinical laboratory·2026
Same journal

Cytomegalovirus Seroprevalence in Morocco - a 10-Year Single-Center Study.

Clinical laboratory·2026
Same journal

Impact of Inactivated SARS-CoV-2 Vaccines on Serum Glycan Profiles and Protein N-Glycosylation.

Clinical laboratory·2026
Same journal

Albumin Concentration Associates Linearly with Unfavorable Outcomes at Three Months Post-Acute Ischemic Stroke in Koreans.

Clinical laboratory·2026
Same journal

Prognostic Value of the Blood Urea Nitrogen-to-Albumin Ratio for ICU Mortality in Cardiogenic Shock Patients: Evidence from the MIMIC-IV Database.

Clinical laboratory·2026
See all related articles

Related Experiment Video

Updated: Mar 23, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.4K

Molecular DNA Analysis in Forensic Identification.

Raluca Dumache, Veronica Ciocan, Camelia Muresan

    Clinical Laboratory
    |March 26, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Forensic genetics utilizes DNA profiling with short tandem repeats (STR) for human identification from biological samples. Advances in molecular biology are introducing new biomarkers like microRNAs for enhanced forensic body fluid identification.

    More Related Videos

    Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
    11:49

    Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

    Published on: March 9, 2015

    16.8K
    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
    07:40

    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

    Published on: March 13, 2011

    21.5K

    Related Experiment Videos

    Last Updated: Mar 23, 2026

    Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
    08:35

    Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

    Published on: July 17, 2021

    23.4K
    Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
    11:49

    Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

    Published on: March 9, 2015

    16.8K
    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
    07:40

    A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

    Published on: March 13, 2011

    21.5K

    Area of Science:

    • Forensic Science
    • Molecular Biology
    • Genetics

    Background:

    • Traditional forensic identification methods (serological, biochemical) suffer from long processing times, low sensitivity, and specificity.
    • DNA profiling, particularly using short tandem repeats (STR), has become a cornerstone of forensic investigations over the past 30 years.
    • STR analysis aids in human identification from various biological samples.

    Purpose of the Study:

    • To review the current state and advancements in forensic genetic identification methods.
    • To highlight the importance of DNA profiling in forensic analysis.
    • To introduce emerging biomarkers for body fluid identification in forensic science.

    Main Methods:

    • Forensic genetics employs polymerase chain reaction (PCR) based analyses.
    • Methods include analysis of autosomal STRs, Y-chromosome STRs, and mitochondrial DNA.
    • These techniques provide crucial information for crime scene investigations and supplementing other identification methods.

    Main Results:

    • The correlation of biological samples at a crime scene with identification, selection, and probative value is a primary consideration in forensic genetic analysis.
    • DNA profiling based on STRs is a key tool for human identification.

    Conclusions:

    • Recent advances in molecular biology have led to the study of novel biomarkers for forensic identification.
    • MicroRNAs (miRNA), messenger RNA (mRNA), and DNA methylation are proposed as new tools for identifying body fluids.
    • These emerging biomarkers hold promise for enhancing forensic identification capabilities.