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Expression of ADAM17 and ADAM10 in nasal polyps
Woo Yong Bae1, Seong Kook Park2, Do Hun Kim2
1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Dong-A University, Busan, Korea.
Background:
The "a disintegrin and metalloproteases" (ADAMs) are a multifunctional gene family that contribute to the homeostasis of the extracellular matrix, transduction of specific intracellular signals, organogenesis, inflammation, tissue remodeling, adhesion, and cell migration. ADAM17 is the best-characterized of the "sheddases," and its putative substrates are widespread, including various inflammatory modulators. ADAM10 is the most similar to ADAM17 in terms of protein sequence and the structural properties of their catalytic domains. The objective of this work was to assess the roles of ADAM17 and ADAM10 in nasal polyps (NPs) by measuring their expression.
Methods:
The expression of ADAM10 and 17 was investigated in NPs at endonasal sinus surgery (n = 15) and compared with that in inferior turbinate mucosa samples obtained from nonallergic hypertrophic rhinitis patients (n = 15). Tissue samples were analyzed by real-time polymerase chain reaction (PCR), Western blotting, and immunohistochemical staining.
Results:
The ADAM17 messenger RNA (mRNA) and protein levels were significantly higher in the inferior turbinate than in NPs (p < 0.05). The ADAM10 mRNA and protein levels did not differ significantly between NPs and inferior turbinates (p > 0.05). ADAM10 and ADAM17 were expressed primarily in inflammatory cells, submucosal glandular cells, and lining epithelial cells.
Conclusion:
ADAM17 may contribute to the development of NPs in contrast to ADAM10, based on their expression patterns. It may be important to discover the role of ADAM17 in the development of NP and helpful to examine the specific mechanism of the development of NPs.
Insights
The study found ADAM17 protein and mRNA levels were lower in nasal polyps (NPs) compared to inferior turbinates. ADAM10 expression showed no significant difference, suggesting ADAM17 may play a role in NP development.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- A disintegrin and metalloproteases (ADAMs) are crucial for extracellular matrix homeostasis, cell signaling, and tissue remodeling.
- ADAM17, a prominent 'sheddase,' influences inflammatory processes through its diverse substrates.
- ADAM10 shares significant structural and sequence similarity with ADAM17.
Purpose of the Study:
- To investigate the expression patterns of ADAM17 and ADAM10 in nasal polyps (NPs).
- To compare the expression levels of ADAM17 and ADAM10 in NPs versus inferior turbinate mucosa.
- To elucidate the potential roles of ADAM17 and ADAM10 in the pathogenesis of nasal polyps.
Main Methods:
- Quantitative analysis of ADAM17 and ADAM10 mRNA and protein levels using real-time PCR and Western blotting.
- Immunohistochemical staining to determine the cellular localization of ADAM10 and ADAM17.
- Comparative study involving nasal polyp tissues (n=15) and inferior turbinate mucosa from non-allergic patients (n=15).
Main Results:
- ADAM17 mRNA and protein levels were significantly lower in nasal polyps compared to inferior turbinates (p < 0.05).
- No significant difference in ADAM10 mRNA and protein levels was observed between nasal polyps and inferior turbinates (p > 0.05).
- Both ADAM10 and ADAM17 were predominantly expressed in inflammatory cells, submucosal glands, and epithelial lining cells.
Conclusions:
- The distinct expression patterns suggest ADAM17, rather than ADAM10, may be implicated in the development of nasal polyps.
- Further research into the specific mechanisms of ADAM17 in NP pathogenesis is warranted.
- Understanding ADAM17's role could provide insights into novel therapeutic strategies for nasal polyps.
