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Updated: Mar 23, 2026
10:47
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
Published on: March 24, 2016
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msIQuant--Quantitation Software for Mass Spectrometry Imaging Enabling Fast Access, Visualization, and Analysis of
Patrik Källback1, Anna Nilsson1, Mohammadreza Shariatgorji1
1Biomolecular Imaging and Proteomics, National Resource for Mass Spectrometry Imaging, Department of Pharmaceutical Biosciences, Uppsala University , Box 591, SE-75124 Uppsala, Sweden.
Analytical Chemistry
|March 26, 2016
Summary
msIQuant is a new software for mass spectrometry imaging analysis. It handles large datasets and quantifies molecules in tissues, aiding drug and neurotransmitter research.
Area of Science:
- * Analytical Chemistry
- * Molecular Imaging
- * Bioinformatics
Background:
- * Mass spectrometry imaging (MSI) generates large, complex datasets.
- * Existing software often lacks manufacturer independence and efficient large-data handling.
- * Quantitative analysis of endogenous compounds and drugs in tissues is challenging.
Purpose of the Study:
- * To introduce msIQuant, a novel, instrument- and manufacturer-independent MSI software suite.
- * To enable efficient analysis and visualization of very large MSI datasets (>50 GB).
- * To provide robust quantitative analysis of analytes in tissue regions of interest.
Main Methods:
- * Development of msIQuant software utilizing the imzML data format.
- * Implementation of rapid image display and analysis without data reduction.
- * Integration of advanced visualization tools (interpolation, transparency, fusion).
- * Automated generation of calibration curves for analyte quantitation.
- * Analysis of regions-of-interest based on various intensity metrics.
Main Results:
- * msIQuant successfully processes and visualizes very large MSI datasets.
- * The software enables accurate quantitation of small molecules like drugs and neurotransmitters.
- * Demonstrated utility in analyzing specific tissue regions and generating reports.
- * Provided tools for enhanced image visualization and data export.
Conclusions:
- * msIQuant is a powerful, versatile tool for MSI data evaluation.
- * Facilitates quantitation of drugs and endogenous compounds in specific tissue areas.
- * Simplifies postprocessing and report creation for MSI studies.
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