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A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology
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A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.

Charlotte Grenade1, Nicolas Moniotte2,3, Eric Rompen4

  • 1Dental Biomaterials Research Unit (d-BRU) and Department of Fixed Prosthodontics, Institute of Dentistry, University of Liège (ULg) and University of Liège Hospital (CHU), Quai Godefroid Kurth 45, 4020, Liège, Belgium. charlotte.grenade@chu.ulg.ac.be.

Cytotechnology
|March 27, 2016
PubMed
Summary

This study introduces two novel insert-based systems (IBS-R and IBS-F) for reliable in vitro cell culture on various biomaterials. These systems improve cell retention and enable comprehensive analysis of cell behavior on rigid and permeable materials.

Keywords:
BiomaterialsCytocompatibilityDental prosthesesHuman gingival fibroblastsInsertMembranes

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • In vitro studies are crucial for assessing biomaterial biological properties.
  • Current cell culture methods face challenges with cell retention and observing both sides of permeable materials.

Purpose of the Study:

  • To develop reliable in vitro methods for studying cell behavior on rigid and flexible/permeable biomaterials.
  • To create two specific insert-based systems (IBS-R and IBS-F) for enhanced cell culture analysis.

Main Methods:

  • IBS-R: Evaluated human gingival fibroblasts (HGFs) attachment, proliferation, and morphology on titanium and glass-ceramic discs using MTS assays and fluorescent microscopy.
  • IBS-F: Assessed HGFs viability and membrane barrier effects on guided bone regeneration membranes via MTS assays and scanning electron microscopy.

Main Results:

  • Both IBS-R and IBS-F facilitated easy handling, improved cell retention, and allowed accurate evaluation of cell proliferation, spreading, and viability.
  • IBS-F enabled the study of cell migration through membranes, with access to both biomaterial faces and culture well bottoms.

Conclusions:

  • The developed insert-based systems (IBS-R and IBS-F) offer a reliable and versatile solution for in vitro biomaterial research.
  • These systems overcome limitations in current cell culture techniques, enhancing the study of cell-biomaterial interactions.