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miR-31 is distinctively overexpressed in primary male extramammary Paget's disease
Hao Guo1, Rui-Qun Qi1, Ya-Ni Lv1
1Department of Dermatology, No.1 Hospital of China Medical University, Shenyang, China.
Abstract:
MicroRNAs (miRNAs) are small noncoding RNAs involved in cancer development. Extramammary Paget's disease (EMPD) is a rare cutaneous malignancy and the role of miRNAs in EMPD remains unknown. Here, we used TaqMan miRNA arrays to characterize miRNA expression profile in EMPD and further validated the candidates by single RT-PCR. Total 12 cases EMPD were involved in this study. Using laser capture micro-dissection technique, we collected EMPD tumor cells (ET, n=12), normal epidermal cells (NE, n=12) and normal apocrine glands cells (NA, n=7). MiRNA arrays from two pairs of ET and corresponding NE showed that miR-375, miR-10b, miR-31, miR-31* were differentially expressed. The single real-time PCR (RT-PCR) further confirmed that miR-375, miR-31 and miR-31* were upregulated in EMPD cells than those of the normal epidermis and apocrine glands. Our preliminary study suggested that these miRNAs could be involved in EMPD development and miR-31 may serve as potential biomarkers of EMPD.
Insights
This study investigated microRNAs (miRNAs) in Extramammary Paget's disease (EMPD), a rare skin cancer. Researchers found specific miRNAs, including miR-31, are upregulated in EMPD, suggesting their role in disease development and potential as biomarkers.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are small noncoding RNAs crucial in regulating gene expression and implicated in various cancers.
- Extramammary Paget's disease (EMPD) is a rare adenocarcinoma of the skin with an unknown miRNA involvement.
- Understanding miRNA profiles in EMPD is essential for elucidating its pathogenesis.
Purpose of the Study:
- To characterize the miRNA expression profile in Extramammary Paget's disease (EMPD).
- To identify specific miRNAs that are differentially expressed in EMPD tumor cells compared to normal tissues.
- To explore the potential of identified miRNAs as diagnostic or prognostic biomarkers for EMPD.
Main Methods:
- Laser capture micro-dissection was employed to isolate EMPD tumor cells (ET) and corresponding normal epidermal (NE) and apocrine gland (NA) cells.
- TaqMan miRNA arrays were utilized for high-throughput miRNA expression profiling.
- Single real-time quantitative polymerase chain reaction (RT-PCR) was performed for validation of array findings.
Main Results:
- MiRNA array analysis revealed differential expression of miR-375, miR-10b, miR-31, and miR-31* between EMPD tumor cells and normal epidermal cells.
- RT-PCR validation confirmed significant upregulation of miR-375, miR-31, and miR-31* in EMPD cells compared to both normal epidermis and apocrine glands.
- These findings indicate a distinct miRNA signature in EMPD.
Conclusions:
- The study identifies specific upregulated miRNAs (miR-375, miR-31, miR-31*) in Extramammary Paget's disease.
- These miRNAs are potentially involved in the development and progression of EMPD.
- miR-31 shows promise as a potential biomarker for EMPD diagnosis.
