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Unfermented grape juice reduce genomic damage on patients undergoing hemodialysis

Zuray Corredor1, Lara Rodríguez-Ribera1, Elisabeth Coll2

  • 1Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici C, Universitat Autònoma de Barcelona, 08193 Bellaterra, Cerdanyola del Vallès, Spain.

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|March 27, 2016
PubMed

Insights

Unfermented grape juice (UGJ) supplementation significantly reduced oxidative DNA damage in hemodialysis chronic kidney disease (HD-CKD) patients. This antioxidant intervention offers potential benefits for managing genotoxicity in this vulnerable population.

Area of Science:

  • Nephrology
  • Oxidative Stress Research
  • Nutritional Science

Background:

  • Patients with chronic kidney disease (CKD) undergoing hemodialysis (HD) experience continuous oxidative stress.
  • This oxidative stress contributes to elevated DNA damage levels in HD-CKD patients.
  • Polyphenols are recognized for their antioxidant properties, suggesting potential therapeutic use.

Purpose of the Study:

  • To investigate the antigenotoxic effects of unfermented grape juice (UGJ) in hemodialysis chronic kidney disease (HD-CKD) patients.
  • To assess whether UGJ supplementation can mitigate DNA damage and oxidative stress markers.

Main Methods:

  • A six-month study involving 39 HD-CKD patients, with 25 receiving UGJ supplementation and 14 serving as a control group.
  • DNA damage was quantified using the comet assay (DNA breaks, oxidized bases) and micronucleus test (chromosome damage).
  • Antioxidant capacity was evaluated using the Trolox equivalent antioxidant capacity (TEAC) assay.

Main Results:

  • UGJ supplementation led to a significant reduction in oxidative DNA damage levels.
  • LDL cholesterol and cholesterol levels decreased significantly in the supplemented group.
  • Cholesterol levels also showed a decrease in the non-supplemented group, but the reduction in DNA damage was specific to the UGJ group.

Conclusions:

  • Supplementation with unfermented grape juice (UGJ) effectively reduced oxidative DNA damage in the studied HD-CKD patients.
  • UGJ demonstrates potential as an intervention to combat genotoxicity associated with oxidative stress in dialysis patients.
  • Further research may explore UGJ's role in improving clinical parameters and overall health outcomes in CKD patients.
Keywords:
Comet assayHemodialysisMicronucleus assayOxidative damageTrolox equivalent antioxidant capacity (TEAC) assayUnfermented grape juice (UGJ)

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