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NMR studies of body fluids
J D Bell1, J C Brown, P J Sadler
1Department of Chemistry, Birkbeck College, University of London, UK.
NMR in Biomedicine
|December 1, 1989
Summary
High-resolution proton nuclear magnetic resonance (NMR) spectroscopy of various body fluids offers powerful diagnostic capabilities. This technique aids in detecting metabolic disorders, drug metabolism, and organ damage, with potential for widespread use in pathology labs.
Area of Science:
- Biochemistry
- Medical Diagnostics
- Analytical Chemistry
Background:
- Proton nuclear magnetic resonance (NMR) spectroscopy provides detailed molecular information.
- Analysis of complex biological matrices like body fluids presents analytical challenges.
Purpose of the Study:
- To describe and discuss high-resolution, high-frequency, in vitro proton NMR spectra of diverse human body fluids.
- To highlight the diagnostic and investigative applications of this spectroscopic technique.
Main Methods:
- Acquisition of proton NMR spectra from various body fluids: urine, sweat, aqueous humour, amniotic fluid, seminal plasma, cerebrospinal fluid, synovial fluid, and blood plasma.
- Utilizing high resolution and high frequency for detailed spectral analysis.
Main Results:
- Demonstrated straightforward metabolite concentration determination in low-protein fluids like urine.
- Showcased detection of small molecules and macromolecule interactions in high-protein fluids (e.g., blood plasma).
- Illustrated lactate-protein interactions in blood plasma.
Conclusions:
- Proton NMR spectroscopy is a versatile tool for analyzing body fluid composition.
- The technique holds significant potential for diagnosing metabolic disorders, understanding drug metabolism, and identifying organ damage.
- These methods are poised for integration into routine pathology laboratory diagnostics.