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Related Experiment Video

Updated: Mar 23, 2026

Fabrication of Amyloid-&#946;-Secreting Alginate Microbeads for Use in Modelling Alzheimer's Disease
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Phages in modified alginate beads.

Farzaneh Moghtader1,2, Sinan Eğri3,4, Erhan Piskin2,4

  • 1a Division of Nanotechnology and Nanomedicine , Institute of Graduate Studies, Hacettepe University , Ankara , Turkey.

Artificial Cells, Nanomedicine, and Biotechnology
|March 29, 2016
PubMed
Summary

Encapsulating T4 phages in alginate beads and coating with chitosan or polyethylene imine (PEI) enhanced phage stability and controlled release. PEI coating offered superior protection against simulated gastric fluid and bile salts.

Keywords:
Alginate beadschitosanencapsulationphagespolyethylene iminestability and sustained release

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Area of Science:

  • Biotechnology
  • Materials Science
  • Microbiology

Background:

  • Bacteriophages (T4 phages) are viruses that infect bacteria.
  • Alginate beads are commonly used for encapsulating biological materials.
  • Chitosan and polyethylene imine (PEI) are cationic polymers with potential for coating and stabilization.

Purpose of the Study:

  • To enhance the stability and control the release of T4 phages.
  • To evaluate the efficacy of alginate bead encapsulation and polycationic coatings (chitosan, PEI).
  • To assess phage viability and release profiles in simulated physiological fluids.

Main Methods:

  • T4 phages were encapsulated in alginate beads.
  • The alginate beads were coated with chitosan and polyethylene imine (PEI).
  • Encapsulated phages were tested for stability in simulated gastric fluid and bile salts, and release kinetics were studied in simulated intestinal fluid.

Main Results:

  • High loading capacities (over 90%) were achieved in pH-sensitive alginate beads.
  • Polycationic coatings significantly increased phage stability, particularly PEI.
  • Phages were released in simulated intestinal fluid at basic pH, with PEI showing slower release than chitosan.

Conclusions:

  • Alginate bead encapsulation combined with polycationic coating effectively stabilizes T4 phages.
  • PEI is a more effective coating material than chitosan for enhancing phage stability and controlling release.
  • This method holds promise for oral phage therapy applications.