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Related Concept Videos

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Alternative RNA Splicing02:18

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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The reciprocal regulation between splicing and 3'-end processing.

Daisuke Kaida1

  • 1Frontier Research Core for Life Sciences, University of Toyama, Toyama, Japan.

Wiley Interdisciplinary Reviews. RNA
|March 29, 2016
PubMed
Summary

RNA processing events like splicing and 3'-end processing are interconnected. The U1 snRNP complex regulates polyadenylation, ensuring transcriptome integrity and controlling gene length.

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Eukaryotic precursor mRNAs undergo essential RNA processing: 5'-end capping, splicing, and 3'-end processing.
  • Historically studied in isolation, these events are now known to be coordinated by interacting processing factors.
  • The U1 small nuclear ribonucleoprotein particle (snRNP) plays a crucial role in regulating these processes.

Purpose of the Study:

  • To elucidate the reciprocal regulatory mechanisms between splicing and 3'-end processing.
  • To highlight the significance of interactions between RNA processing factors for transcriptome integrity.
  • To underscore the role of U1 snRNP in controlling polyadenylation and gene expression.

Main Methods:

  • Review of existing literature and research findings.

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  • Analysis of protein-protein interactions between splicing and 3'-end processing factors.
  • Examination of the functional consequences of these interactions on gene expression.

    • Main Results:

    • U1 snRNP and its components negatively regulate precursor mRNA polyadenylation.
    • Interactions between splicing and 3'-end processing factors across the last exon modulate these processes.
    • These regulatory interactions are crucial for maintaining transcriptome integrity, controlling gene length, and directing transcription.

    Conclusions:

    • Splicing and 3'-end processing are intricately and reciprocally regulated through a complex network of protein-protein interactions.
    • Understanding these coordinated mechanisms is vital for comprehending gene expression control.
    • Further research is needed to fully unravel the molecular details of this reciprocal regulation.