Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intraoperative Zoledronic Acid for Arthroscopic Rotator Cuff Repair Results in Distinct Circulating Micro-RNA Profiles Indicating Improved Tendon-to-Bone Healing.

The American journal of sports medicine·2026
Same author

Transcription-independent induction of rapid-onset senescence is integral to healing.

Nature cell biology·2026
Same author

Clinical outcomes with oral cephalosporins as definitive treatment of Enterobacterales bacteremia from a urinary source based on cefazolin minimum inhibitory concentration.

Antimicrobial agents and chemotherapy·2026
Same author

99th percentile upper reference limits of the 5th and 6th generation Elecsys<sup>®</sup> cardiac troponin T high-sensitivity assays in a single cohort of healthy blood donors.

Clinical chemistry and laboratory medicine·2026
Same author

Interaction of pulsed low frequency electromagnetic field (PEMF) with mitochondria.

Scientific reports·2026
Same author

Intraoperative Zoledronic Acid for Arthroscopic Rotator Cuff Repair: Short-Term Results From a Prospective, Randomized, Placebo-Controlled Phase II Trial.

The American journal of sports medicine·2026

Related Experiment Video

Updated: Mar 23, 2026

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.6K

A Luciferase-Based Quick Potency Assay to Predict Chondrogenic Differentiation.

Eleni Oberbauer1,2, Carolin Steffenhagen1,2, Georg Feichtinger1,2,3

  • 11 Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center , Linz/Vienna, Austria .

Tissue Engineering. Part C, Methods
|March 29, 2016
PubMed
Summary
This summary is machine-generated.

A new bioassay rapidly assesses cartilage repair potential in adipose-derived stem cells (ASCs). This quick potency test, using a novel reporter gene, ensures cell therapy efficacy by detecting chondrogenic potential in just three days.

More Related Videos

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.7K
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
10:07

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Published on: May 17, 2017

17.6K

Related Experiment Videos

Last Updated: Mar 23, 2026

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.6K
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.7K
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
10:07

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

Published on: May 17, 2017

17.6K

Area of Science:

  • Biotechnology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Chondrogenic differentiation of adipose-derived stem cells (ASCs) is crucial for cartilage repair but faces challenges due to donor variability.
  • Assessing the chondrogenic potential of ASCs is vital for ensuring effective cell therapies, but current methods are time-consuming.

Purpose of the Study:

  • To develop a novel, rapid bioassay for quantifying the chondrogenic potential of ASCs.
  • To enable quick assessment of ASC potency for predicting suitability in cell-based cartilage repair therapies.

Main Methods:

  • Human primary ASCs were co-transfected with a Metridia luciferase-based collagen type II reporter gene and a Renilla control plasmid.
  • Chondrogenic potential was analyzed by measuring luciferase activity after 3 days of induction under 3D and 2D culture conditions.

Main Results:

  • The developed bioassay successfully detected chondrogenic differentiation in ASCs from all tested donors after 3 days.
  • Luciferase activity was induced under both three-dimensional (3D) and two-dimensional (2D) culture conditions, demonstrating assay versatility.

Conclusions:

  • A novel quick potency bioassay allows for the determination of chondrogenic potential in ASCs within 3 days.
  • This assay, particularly under 2D culture conditions, significantly enhances the efficiency of testing cell functionality for future cell therapies.