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Updated: Mar 23, 2026
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
Published on: December 10, 2013
[Measles virus stimulation effect on the expression of Toll-like receptors in PBMC]
Introduction:
Toll-like receptors (TLRs) are an important component of a innate immune system. Stimulation of TLRs, through action with helper T cells, could change Th1/Th2 balance and thus affect adaptive immune response. Receptors TLR2 and TLR4 play important role in immune response to measles virus. The aim of this work was stimulation of the peripheral blood mononuclear cells (PBMC) and determination of Toll-like gene expression in these cells.
Methods:
PBMCs from 20 healthy donors were stimulated with measles viruses and ligands for TLR2 and TLR4. For examinations the real time RT-PCR (QuantiFast® Assay, Qiagen) was used. The expression of Toll-like receptors was determined on muRNA level, using real-time one step RT-PCR (QuantiFast® Assay, Qiagen) with simultaneous detection of TLR2 and TLR4 genes and housekeeping gene (GAPDH).
Results:
Virus-specific influence of wild measles virus strains activity on PBMC derived from vaccinated seronegative individuals manifested in higher level of expression of TLR2 and TLR4 genes in compare to the expression of these genes in PBMC of seropositive individuals.
Conclusions:
Toll-like receptors participate in the development of immune response to measles virus.
Insights
Toll-like receptors (TLRs) are key in the immune response to measles virus. Measles virus stimulation increased TLR2 and TLR4 gene expression in peripheral blood mononuclear cells (PBMCs) from vaccinated, seronegative individuals.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Context:
- Toll-like receptors (TLRs) are crucial innate immune sensors.
- TLR stimulation influences adaptive immunity by modulating T-helper cell balance.
- TLR2 and TLR4 are implicated in the immune response to measles virus.
Purpose:
- To investigate the expression of Toll-like receptors (TLRs) in peripheral blood mononuclear cells (PBMCs).
- To determine the effect of measles virus and TLR2/TLR4 ligands on PBMC Toll-like gene expression.
Summary:
- Peripheral blood mononuclear cells (PBMCs) from 20 healthy donors were stimulated with measles virus and TLR2/TLR4 ligands.
- Real-time RT-PCR was used to quantify Toll-like receptor gene expression (TLR2, TLR4) at the mRNA level.
- Higher TLR2 and TLR4 gene expression was observed in PBMCs from vaccinated, seronegative individuals exposed to wild measles virus strains compared to seropositive individuals.
Impact:
- Demonstrates the role of Toll-like receptors in the immune response to measles virus.
- Highlights differential TLR expression based on vaccination and serostatus.
- Provides insights into the innate immune mechanisms underlying measles virus recognition.
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